gfp tfeb expression vector Search Results


human tfe3 gene lentiviral orf cdna expression plasmid, c-gfpspark tag  (Sino Biological)
N/A
Full length Clone DNA of Homo sapiens transcription factor binding to IGHM enhancer 3.
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gfp tfeb expression vector  (Addgene inc)
94
Addgene inc gfp tfeb expression vector
FIGURE 6 MTMR4 knockdown inhibits starvation-induced increase in dephosphorylated <t>TFEB/total</t> TFEB ratio. (a) Phosphorylation of TFEB and other proteins and total amounts of MTMR4 and MTMR3 in control and MTMR4-depleted cells. A549 cells transfected with control- or MTMR4-siRNAs were placed in growth medium (fed) with or without 100 μM chloroquine or in HBSS for 4 hr, and subjected to western blot analyses. S6K, S6 kinase. (b) Quantified data of total TFEB protein level (sum of the dephosphorylated and phosphorylated forms of TFEB) (left), phospho-TFEB level (normalized to GAPDH) (middle) and ratio of dephosphorylated TFEB/total TFEB (right) in control and MTMR4–depleted cells. (c) Time course of starvation-induced change in phosphorylation of TFEB and S6K. A549 cells were starved by culture in HBSS for the indicated time periods and subjected to western blot analyses. Stv, starved. (d) Phosphorylation of TFEB in control and MTMR4-depleted cells under both fed and starved conditions with or without cyclosporine A treatment. Control and MTMR4-depleted A549 cells were incubated in growth medium (fed) or HBSS (starved) with or without cyclosporine-A (10 μM) for 4 hr
Gfp Tfeb Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gfp+tfeb+expression+vector/pm29962048-230-0-13?v=Addgene+inc
Average 94 stars, based on 1 article reviews
gfp tfeb expression vector - by Bioz Stars, 2026-08
94/100 stars
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vectors expressing gfp tfeb  (Addgene inc)
96
Addgene inc vectors expressing gfp tfeb
FIGURE 6 MTMR4 knockdown inhibits starvation-induced increase in dephosphorylated <t>TFEB/total</t> TFEB ratio. (a) Phosphorylation of TFEB and other proteins and total amounts of MTMR4 and MTMR3 in control and MTMR4-depleted cells. A549 cells transfected with control- or MTMR4-siRNAs were placed in growth medium (fed) with or without 100 μM chloroquine or in HBSS for 4 hr, and subjected to western blot analyses. S6K, S6 kinase. (b) Quantified data of total TFEB protein level (sum of the dephosphorylated and phosphorylated forms of TFEB) (left), phospho-TFEB level (normalized to GAPDH) (middle) and ratio of dephosphorylated TFEB/total TFEB (right) in control and MTMR4–depleted cells. (c) Time course of starvation-induced change in phosphorylation of TFEB and S6K. A549 cells were starved by culture in HBSS for the indicated time periods and subjected to western blot analyses. Stv, starved. (d) Phosphorylation of TFEB in control and MTMR4-depleted cells under both fed and starved conditions with or without cyclosporine A treatment. Control and MTMR4-depleted A549 cells were incubated in growth medium (fed) or HBSS (starved) with or without cyclosporine-A (10 μM) for 4 hr
Vectors Expressing Gfp Tfeb, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gfp+tfeb+expression+vector/pmc04590602-356-0-3?v=Addgene+inc
Average 96 stars, based on 1 article reviews
vectors expressing gfp tfeb - by Bioz Stars, 2026-08
96/100 stars
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phage nf kb ta luc ubc gfp w vector  (Addgene inc)
93
Addgene inc phage nf kb ta luc ubc gfp w vector
FIGURE 6 MTMR4 knockdown inhibits starvation-induced increase in dephosphorylated <t>TFEB/total</t> TFEB ratio. (a) Phosphorylation of TFEB and other proteins and total amounts of MTMR4 and MTMR3 in control and MTMR4-depleted cells. A549 cells transfected with control- or MTMR4-siRNAs were placed in growth medium (fed) with or without 100 μM chloroquine or in HBSS for 4 hr, and subjected to western blot analyses. S6K, S6 kinase. (b) Quantified data of total TFEB protein level (sum of the dephosphorylated and phosphorylated forms of TFEB) (left), phospho-TFEB level (normalized to GAPDH) (middle) and ratio of dephosphorylated TFEB/total TFEB (right) in control and MTMR4–depleted cells. (c) Time course of starvation-induced change in phosphorylation of TFEB and S6K. A549 cells were starved by culture in HBSS for the indicated time periods and subjected to western blot analyses. Stv, starved. (d) Phosphorylation of TFEB in control and MTMR4-depleted cells under both fed and starved conditions with or without cyclosporine A treatment. Control and MTMR4-depleted A549 cells were incubated in growth medium (fed) or HBSS (starved) with or without cyclosporine-A (10 μM) for 4 hr
Phage Nf Kb Ta Luc Ubc Gfp W Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gfp+tfeb+expression+vector/pm36708753-343-15-18?v=Addgene+inc
Average 93 stars, based on 1 article reviews
phage nf kb ta luc ubc gfp w vector - by Bioz Stars, 2026-08
93/100 stars
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pcmv-gfp-tfe3pa-del (plasmid #120204)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
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phage-hspc-tfeb(delta30)egfp-w (plasmid #126693)  (Addgene inc)
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Standard format: Plasmid sent in bacteria as agar stab
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pegfp-n1-tfeb-wt-gfp (#845) (plasmid #99958)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
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phage-hspc-tfebegfp-w (plasmid #126692)  (Addgene inc)
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Standard format: Plasmid sent in bacteria as agar stab
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pegfp-n1-tfe3 (plasmid #38120)  (Addgene inc)
N/A
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human tfeb gene orf cdna clone expression plasmid, c-gfpspark tag  (Sino Biological)
N/A
Full length Clone DNA of Human transcription factor EB
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pcmv-gfp-tfe3pa (plasmid #120201)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
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cas9-gfp_sg_mtfeb (plasmid #79006)  (Addgene inc)
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Standard format: Plasmid sent in bacteria as agar stab
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Image Search Results


FIGURE 6 MTMR4 knockdown inhibits starvation-induced increase in dephosphorylated TFEB/total TFEB ratio. (a) Phosphorylation of TFEB and other proteins and total amounts of MTMR4 and MTMR3 in control and MTMR4-depleted cells. A549 cells transfected with control- or MTMR4-siRNAs were placed in growth medium (fed) with or without 100 μM chloroquine or in HBSS for 4 hr, and subjected to western blot analyses. S6K, S6 kinase. (b) Quantified data of total TFEB protein level (sum of the dephosphorylated and phosphorylated forms of TFEB) (left), phospho-TFEB level (normalized to GAPDH) (middle) and ratio of dephosphorylated TFEB/total TFEB (right) in control and MTMR4–depleted cells. (c) Time course of starvation-induced change in phosphorylation of TFEB and S6K. A549 cells were starved by culture in HBSS for the indicated time periods and subjected to western blot analyses. Stv, starved. (d) Phosphorylation of TFEB in control and MTMR4-depleted cells under both fed and starved conditions with or without cyclosporine A treatment. Control and MTMR4-depleted A549 cells were incubated in growth medium (fed) or HBSS (starved) with or without cyclosporine-A (10 μM) for 4 hr

Journal: Genes to cells : devoted to molecular & cellular mechanisms

Article Title: MTMR4, a phosphoinositide-specific 3'-phosphatase, regulates TFEB activity and the endocytic and autophagic pathways.

doi: 10.1111/gtc.12609

Figure Lengend Snippet: FIGURE 6 MTMR4 knockdown inhibits starvation-induced increase in dephosphorylated TFEB/total TFEB ratio. (a) Phosphorylation of TFEB and other proteins and total amounts of MTMR4 and MTMR3 in control and MTMR4-depleted cells. A549 cells transfected with control- or MTMR4-siRNAs were placed in growth medium (fed) with or without 100 μM chloroquine or in HBSS for 4 hr, and subjected to western blot analyses. S6K, S6 kinase. (b) Quantified data of total TFEB protein level (sum of the dephosphorylated and phosphorylated forms of TFEB) (left), phospho-TFEB level (normalized to GAPDH) (middle) and ratio of dephosphorylated TFEB/total TFEB (right) in control and MTMR4–depleted cells. (c) Time course of starvation-induced change in phosphorylation of TFEB and S6K. A549 cells were starved by culture in HBSS for the indicated time periods and subjected to western blot analyses. Stv, starved. (d) Phosphorylation of TFEB in control and MTMR4-depleted cells under both fed and starved conditions with or without cyclosporine A treatment. Control and MTMR4-depleted A549 cells were incubated in growth medium (fed) or HBSS (starved) with or without cyclosporine-A (10 μM) for 4 hr

Article Snippet: GFP- TFEB expression vector (# 38119) was provided by Dr. Shawn Ferguson through Addgene (Roczniak- Ferguson et al., 2012).

Techniques: Knockdown, Phospho-proteomics, Control, Transfection, Western Blot, Incubation

FIGURE 8 PI(3)P-phosphatase MTMR4 regulates endocytic and autophagic pathways. MTMR4 is localized mainly in LEs and APs to regulate PI(3)P levels. MTMR4 regulates the fusion, fission, motility and maturation of endosomes, the autophagic pathway, and lysosomal biogenesis. MTMR4 is also required for activation of TFEB. This action of MTMR4 contributes to MTMR4 regulation of the endocytic and autophagic pathways

Journal: Genes to cells : devoted to molecular & cellular mechanisms

Article Title: MTMR4, a phosphoinositide-specific 3'-phosphatase, regulates TFEB activity and the endocytic and autophagic pathways.

doi: 10.1111/gtc.12609

Figure Lengend Snippet: FIGURE 8 PI(3)P-phosphatase MTMR4 regulates endocytic and autophagic pathways. MTMR4 is localized mainly in LEs and APs to regulate PI(3)P levels. MTMR4 regulates the fusion, fission, motility and maturation of endosomes, the autophagic pathway, and lysosomal biogenesis. MTMR4 is also required for activation of TFEB. This action of MTMR4 contributes to MTMR4 regulation of the endocytic and autophagic pathways

Article Snippet: GFP- TFEB expression vector (# 38119) was provided by Dr. Shawn Ferguson through Addgene (Roczniak- Ferguson et al., 2012).

Techniques: Activation Assay